Measurement

Part:BBa_K549008

Designed by: Tobias Bauer   Group: iGEM11_LMU-Munich   (2011-09-16)


PnorB + RBS + luxAB-reporter genes

The promoter PnorB is regulated by the repressor NorB. In presence of iron (2+) ions the transcription of the reporter gene is activated. In this the case, the reporter is lacZ' (BBa_J33202).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 649
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1168


[edit]
Categories
Parameters
None